
Thermo Fisher Scientific F4/80 Monoclonal Antibody (Cl:A3-1)
Mouse macrophage marker F4/80에 특이적인 Rat monoclonal antibody (Cl:A3-1). 다양한 응용 분야(WB, IHC, Flow Cytometry 등)에 사용 가능. 단백질 G 정제, PBS buffer에 1 mg/mL 농도. 4°C 단기, -20°C 장기 보관 권장.
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Thermo Fisher Scientific F4/80 Monoclonal Antibody (Cl:A3-1)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 1 publication |
| Immunohistochemistry (IHC) | Assay-dependent | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | View 3 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Radioimmune Assays (RIA) | Assay-dependent | - |
| Immunomicroscopy (IM) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Cl:A3-1 |
| Immunogen | Thioglycollate-stimulated peritoneal macrophages from C57/BL mice |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538125 |
Additional Formats:
Product Specific Information
- Paraffin sections require pre-treatment prior to staining.
- Proteinase K is recommended for tissues fixed <24 hrs.
- Citrate buffer (pH 6.0) recommended for tissues fixed >24 hrs.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Rat anti-mouse F4/80 antibody, clone Cl:A3-1 recognizes murine F4/80 antigen (~160 kDa cell surface glycoprotein), a member of the EGF-TM7 family.
- Shares 68% amino acid identity with human EMR1 (EGF module-containing mucin-like hormone receptor 1).
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein belonging to the EGF-TM7 family. It shares 68% identity with human EMR1. Expression varies during macrophage maturation and activation.
F4/80 is expressed on mature tissue macrophages including:
- Kupffer cells
- Langerhans cells
- Microglia
- Macrophages in gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma, and spleen red pulp
It is also found on some dendritic cells but absent from macrophages in T cell areas of spleen and lymph node.
Recent studies suggest a role for F4/80 in generating efferent CD8⁺ regulatory T cells.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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