Thermo Fisher Scientific Phospho-Ephrin B1/B2/B3 (Tyr324) Polyclonal Antibody
Phospho-Ephrin B1/B2/B3 (Tyr324) 폴리클로날 항체로, 인산화된 Ephrin B 단백질의 특이적 검출에 적합합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 사람, 마우스, 랫트 시료에 반응합니다. 고순도 정제 및 글리세롤 보존으로 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EFNB1 (Accession P98172), corresponding to amino acid residues around phosphorylated Tyr324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2815966 |
Product Specific Information
This antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.
Target Information
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis, indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that affect integrin-mediated cell attachment and migration. Its distribution in the developing retina suggests involvement in retinal axon mapping and intratectal development. Ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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