Thermo Fisher Scientific Phospho-Ephrin B1/B2/B3 (Tyr324) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human EFNB1(Accession P98172), corresponding to amino acid residues around phosphorylated Tyr324. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ephrin B1/B2/B3,uniProtId:P98172-1,ncbiNodeId:9606,antigenRange:324,antigenLength:346,antigenImageFileName:PA5-103267_Ephrin_B1B2B3_P98172-1_Rabbit.svg,antigenImageFileNamePDP:PA5-103267_Ephrin_B1B2B3_P98172-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2815966

Product Specific Information

Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.

Target Information

Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.