Thermo Fisher Scientific RANBP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 201 of Ran BP1 (Uniprot ID#P43487) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RANBP1,uniProtId:P43487-1,ncbiNodeId:9606,antigenRange:1-201,antigenLength:201,antigenImageFileName:PA5-21481_RANBP1_P43487-1_Rabbit.svg,antigenImageFileNamePDP:PA5-21481_RANBP1_P43487-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152109

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain.

Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (84%), Pig (96%), Chicken (91%), Rhesus Monkey (99%), Bovine (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.