Takara Calf intestinal alkaline phosphatase (CIAP)

Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5`-phosphate termini from DNA, RNA, dNTPs and rNTPs. This enzyme can be used to reduce linear vector self-ligation and vector background during cloning. CIAP is supplied in a buffer of 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2, and 50% glycerol.

More Information

Applications

• Reduction of linear vector self-ligation and vector background during cloning
• Dephosphorylation of DNA prior to kinase labeling reactions
• Template preparation prior to 5`-end labeling
• Protein dephosphorylation

Source

Yeast

Storage

–20°C

Notes

Calf intestinal alkaline phosphatase (CIAP) cannot be completely heat inactivated, and therefore it is recommended that the DNA product be purified by gel or spin column purification or phenol-chloroform extraction.

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].

Unit definition

One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.

Concentration

10–30 U/µl

Product citations

Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.