Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on all leukocytes and platelets. CD31 mediates homotypic interactions important for adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported:
This antibody is validated for flow cytometric analysis.

Applications Tested:
Tested on mouse thymocytes and splenocytes for flow cytometry. Recommended ≤0.5 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorescence Properties:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs. Expressed ubiquitously within the vascular compartment, mainly at intercellular junctions, CD31 mediates homophilic binding crucial for cell-cell interactions. It plays roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, and thrombosis, functioning as a key vascular regulatory molecule.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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