Thermo Fisher Scientific CD33 Monoclonal Antibody (HIM3-4), APC, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test
• View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• FITC
• PE
• PE-Cyanine5
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.

Applications Reported:
This HIM3-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. It may be used at 5 µL (0.125 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.

Excitation / Emission / Laser:
633–647 nm / 660 nm / Red Laser

Target Information

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules capable of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies. These ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface.
CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia (AML). It is found on granulocyte and macrophage precursors in bone marrow, but not on pluripotent stem cells. CD33 is also expressed on peripheral monocytes and is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias.
Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지