Thermo Fisher Scientific CD33 Monoclonal Antibody (HIM3-4), APC, eBioscience
인간 CD33 항원을 인식하는 HIM3-4 클론의 APC 결합 단일클론 항체. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용. 골수 계통 세포 표지 및 AML 연구에 유용. 4°C 암조건 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): 5 µL (0.125 µg)/test
- View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIM3-4 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811760 |
Additional Formats
Product Specific Information
Description:
The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.
Applications Reported:
This HIM3-4 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
This antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. It may be used at 5 µL (0.125 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.
Excitation / Emission / Laser:
633–647 nm / 660 nm / Red Laser
Target Information
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules capable of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies. These ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface.
CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia (AML). It is found on granulocyte and macrophage precursors in bone marrow, but not on pluripotent stem cells. CD33 is also expressed on peripheral monocytes and is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias.
Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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