Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), APC, eBioscience
Ki-67 단백질을 인식하는 SolA15 클론의 APC 결합 단일클론 항체로, 세포 증식 마커 분석에 적합합니다. Mouse, Rat, Human 등 다양한 종에 반응하며, Flow Cytometry 및 IHC에 사용 가능합니다. 0.06 µg/test로 고감도 검출이 가능합니다.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), APC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg | View 25 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688057 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor Series (450–660)
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0).
Ki-67 is used as a marker for determining the growth fraction of cell populations and is valuable in tumor prognosis studies.
SolA15 also reacts with human, non-human primate, and canine Ki-67.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Recommended usage ≤ 0.06 µg per test (100 µL final volume).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ki-67 is a nuclear protein expressed during G1, S, G2, and M phases, associated with euchromatin and heterochromatin regions.
It serves as a reliable proliferation marker since it is absent in G0 cells and increases through mitosis.
Ki-67 antibodies are widely used to assess cell growth fractions in neoplasms and have strong correlation with tumor grade and prognosis.
Commonly used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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