Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / 570 / 615 / 660
• FITC
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist (345 and 395 kDa) and are expressed in dividing cells. Ki-67 is present in all cell types during active cell cycle phases (G1, S, G2, and mitosis) but absent in resting cells (G0). During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.

In tumor studies, Ki-67 expression serves as a marker to determine the fraction of proliferating cells within a tumor population. This antibody also recognizes canine Ki-67.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and protocol.
Refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins.

• Recommended use: 5 µL (0.25 µg) per test
• A test = antibody amount to stain 100 µL of cell sample
• Cell number range: 10⁵–10⁸ cells/test
• Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ki-67 is a nuclear protein expressed during G1, S, G2, and M phases of the cell cycle. It contains a forkhead-associated (FHA) domain that associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus. Ki-67 shows phase-dependent localization: perinucleolar (G1), nuclear matrix (G2), and chromosomal (M phase).

Ki-67 is widely used as a proliferation marker since it is absent in G0 cells but increases from G1 through mitosis. It helps determine the growth fraction in neoplasms, correlating with tumor grade and prognosis. It is also used as a neuronal proliferation marker.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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