Thermo Fisher Scientific Phospho-Caspase 9 (Ser196) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Caspase 9 only when phosphorylated at Ser196.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain.
Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis through proteolysis of specific substrates. Caspase 9 is active as a heterotetramer and translocates from the cytosol to mitochondria upon activation.
It plays a central role in the caspase activation cascade responsible for apoptosis execution, cleaving and activating Caspase 3 and Caspase 6.
Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP).
It is expressed in various human tissues. Mutations in Caspase 9 can affect brain development, and insufficient expression in mice results in abnormal cerebrum and neuron development.
In humans, altered Caspase 9 expression is associated with cancer and neurodegenerative diseases such as Alzheimer’s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.