Thermo Fisher Scientific Securin Recombinant Rabbit Monoclonal Antibody (19H16L48)
Securin 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 면역세포염색(ICC/IF)에 적합합니다. 높은 특이성과 재현성을 제공하며, Human 및 Mouse 시료에 반응합니다. Protein A 정제, PBS 완충액 내 0.09% sodium azide 포함, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 6 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 19H16L48 |
| Immunogen | Recombinant protein corresponding to amino acids 1–202 of Securin (UniProt ID: O95997-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2532345 |
Product Specific Information
- This antibody is predicted to react with bovine based on sequence homology.
- Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction produces ~25 kDa (light chain) and ~50 kDa (heavy chain) bands.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits.
- Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
The encoded Securin protein is a homolog of yeast securin proteins that prevent separins from promoting sister chromatid separation. It acts as an anaphase-promoting complex (APC) substrate, associating with separin until APC activation. This protein exhibits transforming and tumorigenic activity and is highly expressed in various tumors. It contains two PXXP motifs essential for transformation and tumorigenicity, a destruction box (D box) for APC-mediated degradation, and an acidic C-terminal region functioning as a transactivation domain. Securin is mainly cytosolic but partially localizes in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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