
Thermo Fisher Scientific PIWIL1 Recombinant Rabbit Monoclonal Antibody (10H15L2)
PIWIL1 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, Western blot과 Immunocytochemistry에 적합합니다. 높은 특이성과 재현성을 제공하며, 인간·마우스·랫트 반응성이 있습니다. 단백질 A로 정제된 액상 항체로 연구용입니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10H15L2 |
| Immunogen | Recombinant protein corresponding to amino acids 90–213 of human Hiwi (PIWIL1, UniProt ID: Q96J94-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532421 |
Product Specific Information
- Predicted to react with mouse based on sequence homology.
- On non-reducing gel, intact IgG appears as ~150 kDa band; upon reduction, generates ~25 kDa light chain and ~50 kDa heavy chain bands.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning antibody DNA sequences from immunoreactive rabbits.
- Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Hiwi (PIWIL1) is a member of the Piwi/Argonaute protein family.
It is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis.
Hiwi is also expressed in CD34⁺ hematopoietic progenitors, but not in CD34⁻ hematopoietic cells, suggesting a role in maintaining hematopoietic stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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