Thermo Fisher Scientific ERK1 Recombinant Rabbit Monoclonal Antibody (3H4L13)
ERK1 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 높은 특이도와 재현성을 제공하며, lot 간 일관성이 우수함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View publication |
| Flow Cytometry (Flow) | 2–4 µg/1×10⁶ cells | View publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3H4L13 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532425 |
Product Specific Information
- Intact IgG appears on a non-reducing gel as ~150 kDa band, and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Individual clones are screened to select the best candidates for production.
- Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway.
ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells.
It responds to stimuli such as growth factors, cytokines, viral infection, GPCR ligands, and transforming agents.
Functionally, ERK1 participates in signaling cascades that control proliferation, differentiation, and cell cycle progression.
Activated ERK1 translocates to the nucleus to phosphorylate nuclear targets.
Alternatively spliced transcript variants encoding different ERK1 isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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