Thermo Fisher Scientific MBD2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (RNDPLNQNKGKPDLN) corresponding to the internal amino acids of MBD2 (aa 226-241). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MBD2,uniProtId:Q9UBB5-1,ncbiNodeId:9606,antigenRange:226-241,antigenLength:411,antigenImageFileName:PA5-18619_MBD2_Q9UBB5-1_Goat.svg,antigenImageFileNamePDP:PA5-18619_MBD2_Q9UBB5-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10977203

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Target Information

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double- primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.