Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), eBioscience

Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 expression in B-1 cells characterizes a distinct lineage of B cells. CD5 acts as a counter-receptor for CD72 and plays a role in T-B cell interaction.

Applications Reported:
Flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD5 is a 67 kDa transmembrane glycoprotein found on all mature T-lymphocytes, most thymocytes, and many T-cell leukemias and lymphomas. It also reacts with a subpopulation of activated B-cells and may regulate T-cell proliferation. CD5 is expressed on 95% of thymocytes and 72% of peripheral blood lymphocytes, primarily in T cell areas of lymph nodes. Associated diseases include thymus cancer and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.