Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells, and a subset of B cells. CD5 expression on a small subset of B cells characterizes B-1 cells, a distinct lineage involved in immune regulation. CD5 serves as a counter-receptor for CD72 and plays a role in T-B cell interactions.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.2 µg/test (100 µL final volume). Optimal performance requires empirical titration (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample to antibody cocktails before combining with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence signatures, minimizing spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD5 is a 67 kDa single-chain transmembrane glycoprotein found on mature T-lymphocytes, thymocytes, and some B cells. It regulates T-cell proliferation and is expressed in most T-cell leukemias and lymphomas.

• Found on 95% of thymocytes and 72% of peripheral blood lymphocytes.
• Major reactivity in T-cell areas of lymph nodes.
• Associated diseases: thymus cancer, Richter’s Syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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