Thermo Fisher Scientific PIAS1 Polyclonal Antibody
PIAS1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합함. 인간 시료 반응성, 비결합형 액상 형태, 고순도 친화 크로마토그래피 정제, 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 5 µg/mL |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis suggests 92.9% homology to mouse for the immunogen sequence. Cross-reactivity with PIAS1 family and other sources has not been determined.
Target Information
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and its substrate, and acts as a SUMO-tethering factor. It plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT, p53, and steroid hormone signaling pathways.
In testis, PIAS1 acts as a nuclear receptor transcriptional coregulator and may contribute to androgen receptor initiation and spermatogenesis maintenance. Transcriptional effects may vary depending on biological context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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