Takara Human induced pluripotent stem cells

Cellartis Human iPS Cell Line Kits contain human induced pluripotent stem (iPS) cells that are banked and characterized according to the highest industry standards, along with a starter set for the Cellartis DEF-CS Culture System. The iPS cells are adapted to the Cellartis DEF-CS Culture System, an easy-to-use, complete culture system for efficient iPS-cell expansion in a feeder-free, defined environment.

Cellartis Human iPS Cell Line Kits contain human induced pluripotent stem (iPS) cells that are banked and characterized according to the highest industry standards, along with a starter set for the Cellartis DEF-CS Culture System. The iPS cells are adapted to the Cellartis DEF-CS Culture System, an easy-to-use, complete culture system for efficient iPS-cell expansion in a feeder-free, defined environment. With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the DEF-CS system coupled with the stable high growth rate of these stem cell lines is ideal for mass production of cells. We currently offer multiple cell lines derived from different donors (see product image).

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Overview

• Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver OCT-4, SOX2, KLF4, and c-Myc
• Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (OCT-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected karyotype
• Optimized for culture with the Cellartis DEF-CS Culture System
• Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated
• Kits include the Cellartis DEF-CS 100 Culture System

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Source

Cell lines were derived from human skin fibroblasts of different donors. Please refer to the Certificate of Analysis in the Documents tab for cell line-specific information, such as donor information and karyotype.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.