Thermo Fisher Scientific Phospho-PKC alpha (Thr497) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects endogenous levels of PKC alpha only when phosphorylated at Thr497.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, and substrate specificity.

PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.

Each PKC family member has a specific expression profile and distinct cellular roles. PKC alpha has been reported to regulate processes such as cell adhesion, transformation, cell cycle checkpoints, and cell volume control. Knockout studies in mice suggest it is a fundamental regulator of cardiac contractility and Ca²⁺ handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.