Thermo Fisher Scientific Phospho-p130 (Thr642) Polyclonal Antibody
인간 RBL2 단백질의 Thr642 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 적합하며, 세포 분열 조절 및 크로마틴 구조 연구에 활용 가능. 1 mg/mL 액상 형태, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RBL2 around the phosphorylation site of Thr642 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817506 |
Target Information
RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and that of constitutive heterochromatin through stabilization of histone methylation.
RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 Lys20 trimethylation and may also act as a transcription repressor by recruiting chromatin-modifying enzymes to promoters.
RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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