Thermo Fisher Scientific Cul3 Polyclonal Antibody
Cul3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등에 사용 가능. FFPE 조직에서 citrate buffer(pH 6.0) 전처리 권장. Rat, X. laevis, X. tropicalis에서도 반응 예측. 4°C 보관, Wet ice 운송.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 650 and 700 of human cullin 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat, X. laevis, and X. tropicalis.
Target Information
Cullin 3 is a member of the family of human cullin genes (CUL1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc53 gene.
CUL1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc53 (known as CulA), these three subunits form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins.
In contrast, CUL2 does not associate with p19 skp1 and p45 skp2 but forms a complex with an inactive transcriptional elongation complex (SIII) composed of Elongin C, Elongin B, and VHL. The function of this association remains unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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