Thermo Fisher Scientific CD28 Recombinant Mouse Monoclonal Antibody (CD28.2)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• Custom conjugation available upon request

Product Specific Information

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include improved specificity and consistent performance across lots.

Description:
The CD28.2 recombinant mouse monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and acts as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.

Applications Reported:
The CD28.2 antibody has been reported for use in flow cytometric analysis and T cell costimulation assays in vitro.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells at 0.025 µg/test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be empirically determined (typically 10^5–10^8 cells/test). Antibody titration is recommended for optimal performance.

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein and a member of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical costimulatory receptor providing the second activation signal by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering an anergic state or undergoing premature apoptosis. In murine lymphoid organs and blood, all CD4+ and CD8+ T cells express CD28, with expression patterns varying across thymocyte maturation stages. CD28 dysfunction has been associated with diseases such as mycosis fungoides and Sézary’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.