Thermo Fisher Scientific CD28 Recombinant Mouse Monoclonal Antibody (CD28.2)
인간 CD28 항원을 인식하는 재조합 마우스 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. 높은 특이성과 로트 간 일관성을 제공하며, 단기 4°C, 장기 -20°C 보관이 가능합니다. Protein A/G로 정제된 액상 형태로 공급됩니다.
- 카탈로그번호
- 740009Mx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD28 Recombinant Mouse Monoclonal Antibody (CD28.2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.025 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CD28.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2942253 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Custom conjugation available upon request
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include improved specificity and consistent performance across lots.
Description:
The CD28.2 recombinant mouse monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and acts as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.
Applications Reported:
The CD28.2 antibody has been reported for use in flow cytometric analysis and T cell costimulation assays in vitro.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells at 0.025 µg/test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be empirically determined (typically 10^5–10^8 cells/test). Antibody titration is recommended for optimal performance.
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein and a member of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical costimulatory receptor providing the second activation signal by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering an anergic state or undergoing premature apoptosis. In murine lymphoid organs and blood, all CD4+ and CD8+ T cells express CD28, with expression patterns varying across thymocyte maturation stages. CD28 dysfunction has been associated with diseases such as mycosis fungoides and Sézary’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.