Thermo Fisher Scientific Ki-67 Recombinant Rat Monoclonal Antibody (SolA15)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:400-1:10,000

-

Flow Cytometry (Flow)

0.063 µg/test

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

SolA15

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 647
• Biotin
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074181

Product Specific Information

Recombinant Rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.