Thermo Fisher Scientific Ki-67 Recombinant Rat Monoclonal Antibody (SolA15)
Ki-67 단백질을 표적하는 recombinant rat monoclonal antibody로 세포 증식 마커 연구에 적합함. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 높은 특이성과 로트 간 일관성 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific Ki-67 Recombinant Rat Monoclonal Antibody (SolA15)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:400–1:10,000 |
| Flow Cytometry (Flow) | 0.063 µg/test |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074181 |
Available Formats
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 647
- Biotin
- Custom conjugation available upon request
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Specific genes encoding the desired antibodies are cloned into expression vectors and expressed in vitro.
Advantages include improved specificity and consistent performance across lots.
It is recommended to carefully titrate the antibody for optimal assay performance.
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It contains a forkhead-associated (FHA) domain and associates with euchromatin and nucleolar regions.
Expression patterns vary by cell cycle stage: perinucleolar in G1, nuclear matrix in G2, and chromosomal during mitosis.
Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases through mitosis.
In tumor tissues, Ki-67 index correlates with histological grade and serves as a prognostic indicator.
Commonly used as a neuronal marker for cell cycling and proliferation studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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