Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI6D10), TrueMAB

Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI6D10), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:500–1:2,000

Species Reactivity

• Human
• Mouse
• Rat

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps facilitating the conjugation of Ub and Ub-like molecules to specific substrates:

1. Activation: The E1 enzyme activates the Ub C-terminus.
2. Conjugation: The E2 enzyme (including UBE2E3, also known as UBCH9) forms an intermediate Ub–E2 complex.
3. Ligation: The E3 ligase transfers Ub to the target protein.

UBE2E3 catalyzes the covalent attachment of ubiquitin to other proteins and regulates transepithelial sodium transport in renal cells. It may also play a role in cell growth arrest and shuttles between cytoplasm and nucleus in an IPO11-dependent manner. Expression is ubiquitous at low levels and elevated in skeletal muscle.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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