Thermo Fisher Scientific I-A/I-E Monoclonal Antibody (M5/114), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2-3 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M5/114

Immunogen

Activated C57BL/6 mouse spleen cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MHC Class II (I-A/I-E),uniProtId:P01921-1,ncbiNodeId:10090,antigenRange:1-265,antigenLength:265,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P01921-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P01921-1_Rat_PDP.jpeg,sortOrder:1},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P06342-1,ncbiNodeId:10090,antigenRange:1-265,antigenLength:265,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P06342-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P06342-1_Rat_PDP.jpeg,sortOrder:2},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P06343-1,ncbiNodeId:10090,antigenRange:1-263,antigenLength:263,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P06343-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P06343-1_Rat_PDP.jpeg,sortOrder:3},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P06344-1,ncbiNodeId:10090,antigenRange:1-263,antigenLength:263,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P06344-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P06344-1_Rat_PDP.jpeg,sortOrder:4},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P06345-1,ncbiNodeId:10090,antigenRange:1-263,antigenLength:263,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P06345-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P06345-1_Rat_PDP.jpeg,sortOrder:5},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P06346-1,ncbiNodeId:10090,antigenRange:1-252,antigenLength:252,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P06346-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P06346-1_Rat_PDP.jpeg,sortOrder:6},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P14483-1,ncbiNodeId:10090,antigenRange:1-265,antigenLength:265,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P14483-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P14483-1_Rat_PDP.jpeg,sortOrder:7},{targetFamily:MHC Class II (I-A/I-E),uniProtId:P04230-1,ncbiNodeId:10090,antigenRange:1-264,antigenLength:264,antigenImageFileName:MA1-10403_MHC_Class_II_I-AI-E_P04230-1_Rat.svg,antigenImageFileNamePDP:MA1-10403_MHC_Class_II_I-AI-E_P04230-1_Rat_PDP.jpeg,sortOrder:8}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11155427

Product Specific Information

This antibody recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.