Thermo Fisher Scientific LIME Monoclonal Antibody (LIME-06), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1-5 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

LIME-06

Immunogen

Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LIME,uniProtId:Q9H400-1,ncbiNodeId:9606,antigenRange:141-295,antigenLength:295,antigenImageFileName:MA1-10398_LIME_Q9H400-1_House_mouse.svg,antigenImageFileNamePDP:MA1-10398_LIME_Q9H400-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153793

Target Information

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.