Thermo Fisher Scientific Phospho-Synaptotagmin 1/2 (Ser309) Polyclonal Antibody
Synaptotagmin 1/2의 Ser309 인산화 형태를 특이적으로 검출하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. Rabbit IgG 기반으로 고순도 정제. 인간, 마우스, 랫트 반응성. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-Synaptotagmin 1/2 (Ser309) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SYT1 (Accession P21579), corresponding to amino acid residues around phosphorylated Ser309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816312 |
Product Specific Information
This antibody detects endogenous levels of Synaptotagmin 1/2 only when phosphorylated at Serine 309.
Target Information
Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis, and are involved in neurotransmitter secretion from small secretory vesicles.
Calcium binds to Synaptotagmin I, triggering neurotransmitter release at the synapse.
Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions.
Synaptotagmin III participates in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons.
Synaptotagmin IV is expressed in neuronal tissues, with highest mRNA levels in the hippocampus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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