Thermo Fisher Scientific TRIM21 Recombinant Rabbit Monoclonal Antibody (PSH02-56)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH02-56

Immunogen

Recombinant protein within human TRIM21 aa 1-475. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIM21,uniProtId:P19474-1,ncbiNodeId:9606,antigenRange:1-475,antigenLength:475,antigenImageFileName:MA5-52082_TRIM21_P19474-1_Rabbit.svg,antigenImageFileNamePDP:MA5-52082_TRIM21_P19474-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3099396

Product Specific Information

Tissue Specificity: fetal and adult heart and fetal lung.

Positive Control: HeLa cell lysate, HeLa treated with 10 ng/mL IFN-alpha for 16 hours cell lysate, A549 cell lysate, 293T cell lysate, U-2 OS cell lysate, U-2 OS, human spleen tissue, human tonsil tissue.

Subcellular Location: Cytoplasm, Cytoplasmic vesicle, autophagosome, Nucleus, P-body.

Predicted band size: 54 kDa.

Target Information

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.