Thermo Fisher Scientific PTEN Recombinant Rabbit Monoclonal Antibody (SJ19-03)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

SJ19-03

Immunogen

Recombinant full length protein of human pten. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PTEN,uniProtId:P60484-1,ncbiNodeId:9606,antigenRange:1-403,antigenLength:403,antigenImageFileName:MA5-51927_PTEN_P60484-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51927_PTEN_P60484-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093930

Product Specific Information

Sequence Similarities: 99% Mouse/98%Rat.

Tissue Specificity: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Positive Control: MCF7 cell lysate, A431 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Hela, A549, SW480, mouse brain tissue, rat brain tissue.

Subcellular Location: Cytoplasm, Nucleus, Secreted.

Predicted band size: 47 kDa.

Target Information

PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.