Thermo Fisher Scientific GM-CSF Recombinant Rabbit Monoclonal Antibody (7H3L2)

Applications

Western Blot (WB)

• Tested Dilution: 1–3 µg/mL

Product Specifications

Product Specific Information

• Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
• Individual clones are screened to select optimal candidates for production.
• Advantages include:
  • Better specificity and sensitivity
  • Lot-to-lot consistency
  • Animal origin-free formulations
  • Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at chromosome region 5q31, associated with interstitial deletions in 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene promotes tissue inflammation, and elevated cytokine levels, including GM-CSF, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.