Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test
• Tested and validated for flow cytometric analysis
• View 10 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. PD-L1 (B7-H1) and PD-L2 (B7-DC) are known ligands of PD-1 and are upregulated on activated antigen-presenting cells.

Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody required to stain a cell sample in a final volume of 100 µL (10⁵–10⁸ cells/test).

Fluorochrome characteristics:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (Blue laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass (695/40 acceptable alternative)

Fixation stability:
Stained samples remain stable after exposure to 2% formaldehyde overnight at 4°C.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T-cell activation is regulated by PD-1 (PDCD-1), which mediates inhibitory signals upon binding to PD-L1 or PD-L2 on antigen-presenting cells. These interactions help maintain immune tolerance and prevent autoimmunity. PD-1 deficiency leads to hyperactivated immune responses and autoimmune disease. Although PD-1 has a predicted molecular weight of 55 kDa, it may migrate at higher molecular weights in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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