Thermo Fisher Scientific HSP27 Monoclonal Antibody (10-8)
HSP27 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC에 적합합니다. 인간, 마우스, 랫트 시료에서 반응하며, Protein A로 정제된 액상 형태입니다. 세포 스트레스 반응 및 단백질 폴딩 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10-8 |
| Immunogen | Recombinant protein within Human HSP27 aa 11–190/205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS, pH 7.4, with 40% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931076 |
Target Information
Cells from many organisms increase the expression of heat shock or stress proteins in response to environmental stress.
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein functioning as a molecular chaperone that maintains denatured proteins in a folding-competent state.
It plays a role in stress resistance and actin organization, and regulates biological processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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