Thermo Fisher Scientific E-selectin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (IHC)

5-15 µg/mL

-

Neutralization (Neu)

Assay-Dependent

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat E-Selectin/CD62E Trp22-Pro494 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E-selectin (CD62E),uniProtId:P98105-1,ncbiNodeId:10114,antigenRange:22-494,antigenLength:549,antigenImageFileName:PA5-47978_E-selectin_CD62E_P98105-1_Goat.svg,antigenImageFileNamePDP:PA5-47978_E-selectin_CD62E_P98105-1_Goat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608214

Product Specific Information

In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) E-Selectin is observed, approximately 5% cross-reactivity with recombinant human (rh) E-Selectin is observed and less than 1% cross-reactivity with rmL-Selectin and rmP-Selectin is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.