
Thermo Fisher Scientific E-selectin Polyclonal Antibody
Thermo Fisher Scientific의 E-selectin Polyclonal Antibody는 Rat E-selectin/CD62E를 인식하는 Goat IgG 항체로, WB, IHC, Neutralization 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 5% trehalose 버퍼에 보존됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | 5–15 µg/mL |
| Neutralization (Neu) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant rat E-Selectin/CD62E (Trp22–Pro494) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608214 |
Product Specific Information
- In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) E-Selectin, 5% with recombinant human (rh) E-Selectin, and <1% with rmL-Selectin and rmP-Selectin observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. Leukocyte adhesion occurs in capillaries and small venules after inflammation onset, involving adhesion receptor cascades. Selectins, as C-type cell surface lectins, mediate leukocyte adhesion to endothelial surfaces.
E-selectin (CD62E) is expressed by endothelial cells only after activation by proinflammatory cytokines such as IL-1, TNFα, or bacterial components like lipopolysaccharides via an NFκB-dependent pathway.
E-selectin is associated with vascular endothelium in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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