Thermo Fisher Scientific GM-CSF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Neutralization (Neu)

0.5-2.0 µg/mL

-

Product Specifications

Species Reactivity

Cat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant feline GM-CSF

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610127

Product Specific Information

In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse GM-CSF, recombinant rat GM-CSF, and recombinant human GM-CSF is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.