Thermo Fisher Scientific Glucoamylase Polyclonal Antibody
Rhizopus 유래 Glucoamylase를 인식하는 염소 다클론 항체로, WB, ELISA, IP에 적합합니다. 고순도 정제된 IgG 형식으로 제공되며 비결합 상태입니다. 단기 4°C, 장기 -20°C 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Glucoamylase Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fungi |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glucoamylase [Rhizopus] |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-GLUCOAMYLASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography, followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]. Cross reactivity against Glucoamylase from other sources is unknown.
Target Information
Rhizopus glucoamylase exists in multiple forms (Gluc 1, Gluc 2, and Gluc 3), all of which hydrolyze gelatinized starch at similar rates, but only the largest form (Gluc 1) can adsorb raw starch.
It hydrolyzes terminal (1→4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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