Thermo Fisher Scientific Sucrose Phosphorylase Polyclonal Antibody
Thermo Fisher Scientific의 Sucrose Phosphorylase Polyclonal Antibody는 E.coli 유래 효소를 인식하는 염소 IgG 항체로, WB, ELISA, IP에 사용 가능. 고순도 이온교환 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Sucrose Phosphorylase (E.coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Sucrose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase (E.coli).
Cross-reactivity against Sucrose Phosphorylase from other sources is unknown.
Target Information
Sucrose phosphorylase catalyzes the reaction:
Sucrose + phosphate → D-fructose + α-D-glucose 1-phosphate
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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