Thermo Fisher Scientific KEAP1 Monoclonal Antibody (1F10B6)
KEAP1 단백질을 검출하기 위한 Thermo Fisher Scientific의 Mouse 모노클로날 항체. Western blot, ELISA, ICC/IF 등 다양한 응용에 적합. Human 및 Mouse 시료에 반응하며, 고품질의 연구용 항체로 신뢰성 높은 결과 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50 | – |
| Flow Cytometry (Flow) | 1:200–1:400 | – |
| ELISA | 1:10,000 | – |
| in situ PLA (PLA) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F10B6 |
| Immunogen | Purified recombinant fragment of human KEAP1 (amino acids 380–624) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2538577 |
Product Specific Information
MA5-17106 targets KEAP1 in indirect ELISA, FACS, ICC, IF, and WB applications and shows reactivity with Human and Mouse samples.
The immunogen is a purified recombinant fragment of human KEAP1 (amino acids 380–624) expressed in E. coli.
MA5-17106 detects KEAP1, which has a predicted molecular weight of approximately 69.7 kDa.
Target Information
The KEAP1 gene encodes a protein containing KELCH-like domains and a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 (Nrf2) in a redox-sensitive manner. Upon dissociation in the cytoplasm, Nrf2 translocates to the nucleus, leading to the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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