Thermo Fisher Scientific KEAP1 Monoclonal Antibody (1F10B6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

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Immunohistochemistry (Frozen) (IHC (F))

-

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Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F10B6

Immunogen

Purified recombinant fragment of human KEAP1 (amino acids: 380-624) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KEAP1,uniProtId:Q14145-1,ncbiNodeId:9606,antigenRange:380-624,antigenLength:624,antigenImageFileName:MA5-17106_KEAP1_Q14145-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17106_KEAP1_Q14145-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538577

Product Specific Information

MA5-17106 targets KEAP1 in indirect ELISA, FACS, ICC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17106 immunogen is purified recombinant fragment of human KEAP1 (amino acids: 380-624) expressed in E. Coli.

MA5-17106 detects KEAP1 which has a predicted molecular weight of approximately 69.7kDa.

Target Information

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.