Thermo Fisher Scientific KEAP1 Monoclonal Antibody (7G4B10)
Human KEAP1 단백질을 표적으로 하는 마우스 단일클론 항체로, WB, IHC, Flow Cytometry, ELISA에 적합합니다. Protein G로 정제된 액상 형태이며, 1 mg/mL 농도로 제공됩니다. KEAP1 연구 및 산화 스트레스 관련 신호전달 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7G4B10 |
| Immunogen | Purified recombinant fragment of human KEAP1 (amino acids 380–624) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538578 |
Product Specific Information
MA5-17107 targets KEAP1 in FACS, IHC, indirect ELISA, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human KEAP1 (amino acids 380–624) expressed in E. coli.
MA5-17107 detects KEAP1, which has a predicted molecular weight of approximately 69.7 kDa.
Target Information
The KEAP1 gene encodes a protein containing KELCH-like domains and a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 (Nrf2) in a redox-sensitive manner. Upon dissociation in the cytoplasm, Nrf2 translocates to the nucleus, inducing expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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