Thermo Fisher Scientific HNF4A Recombinant Rabbit Monoclonal Antibody (SN72-03)
HNF4A 단백질을 인식하는 토끼 모노클로날 항체로, Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 재조합 항체로 높은 특이성과 재현성을 제공. 인간 및 생쥐 시료에 반응하며, Protein A로 정제된 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:3000 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:1000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN72-03 |
| Immunogen | Synthetic peptide within Human HNF4A (aa 21–70) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809580 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4A) is an orphan nuclear receptor expressed in the liver, kidney, intestine, and pancreas. Mutations in HNF4A are associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4A binds DNA as a homodimer and has multiple isoforms (HNF4A1–HNF4A9) generated by alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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