Thermo Fisher Scientific HNF4A Recombinant Rabbit Monoclonal Antibody (SN72-03)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:3000

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:1000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN72-03

Immunogen

Synthetic peptide within Human HNF-4-alpha aa 21-70 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HNF4A,uniProtId:P41235-1,ncbiNodeId:9606,antigenRange:21-70,antigenLength:474,antigenImageFileName:MA5-32295_HNF4A_P41235-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32295_HNF4A_P41235-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809580

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.