Thermo Fisher Scientific ATG5 Recombinant Rabbit Monoclonal Antibody (SN73-07)
ATG5 단백질을 검출하기 위한 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF에 적합하며 높은 특이성과 민감도를 제공. 로트 간 일관성, 동물 유래 성분 없음, 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN73-07 |
| Immunogen | Synthetic peptide within Human ATG5 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809574 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
ATG5 (Autophagy Related 5) is a key component of autophagy and plays an important role in apoptosis. It is involved in:
- Mitochondrial quality control after oxidative damage
- Negative regulation of innate antiviral immune response
- Lymphocyte development and proliferation
- MHC II antigen presentation and adipocyte differentiation
Following conjugation to ATG12, the complex participates in autophagosome formation. ATG5 contributes to autophagic cell death by interacting with FADD and forms a cup-shaped isolation membrane that detaches before or after autophagosome completion.
ATG5 dysfunction is associated with diseases such as spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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