Thermo Fisher Scientific Phospho-AKT1 (Ser473) Polyclonal Antibody
Phospho-AKT1 (Ser473) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응. Western blot, IHC, ELISA 등에 사용 가능. 액상 형태로 1.52 mg/mL 농도, -20°C 보관. 세포 생존 및 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S473 of human AKT1 (NP_005154.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.52 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914836 |
Product Specific Information
Positive test controls include Jurkat and C6 cells.
The target is typically localized in the cell membrane, cytoplasm, and nucleus.
Immunogen sequence: QFSYS.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival.
The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating multiple targets in various cell types, including cancer cells.
This protein kinase is activated by insulin, PI3K, IGF1, and other growth and survival factors.
Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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