Thermo Fisher Scientific Phospho-PKC alpha/beta-2 (Thr638, Thr641) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T638 of human PKC alpha/beta IIPRKCA (NP_0027282) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC alpha/beta-2,uniProtId:P05771-1,ncbiNodeId:9606,antigenRange:638,antigenLength:671,antigenImageFileName:PA5-121268_PKC_alphabeta-2_P05771-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121268_PKC_alphabeta-2_P05771-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:PKC alpha/beta-2,uniProtId:P17252-1,ncbiNodeId:9606,antigenRange:638,antigenLength:672,antigenImageFileName:PA5-121268_PKC_alphabeta-2_P17252-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121268_PKC_alphabeta-2_P17252-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914840

Product Specific Information

Positive test controls include: NIH/3T3. The target is usually found in the following locations: Cytoplasm, Membrane, Nucleus, Peripheral membrane protein.

Immunogen sequence: VLTPP

Target Information

Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.