Thermo Fisher Scientific Bax Polyclonal Antibody
BAX 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, Flow cytometry에 사용 가능. Human과 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. 세포 사멸 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 6 publications |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 1:25 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 41–76 amino acids from human Bax |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.6 mg/mL |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2061694 |
Product Specific Information
This antibody is predicted to react with bovine based on sequence homology.
Target Information
BAX is a member of the Bcl-2 family and plays a crucial role in the regulation of apoptosis. While Bcl-2 acts as an anti-apoptotic protein, BAX promotes apoptosis. Apoptosis regulation involves both homo- and heterodimerization of various isoforms of BAX and Bcl-2. The Bax gene encodes isoforms such as Bax alpha (21 kDa) and Bax beta (24 kDa), both containing BH1, BH2, and BH3 domains. Bax beta has a unique carboxyl terminus lacking a hydrophobic transmembrane domain. Bcl-2 isoforms differ in C-terminal and UTR sequences. Overexpression of BAX promotes cell death through dimer formation with other BCL-2 family proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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