Thermo Fisher Scientific Renal Cell Carcinoma (Carbonic Anhydrase IX) Monoclonal Antibody (66.4.C2 (PN-15))
Renal Cell Carcinoma (Carbonic Anhydrase IX) 단일클론 항체로, 인간 및 말 조직 반응성. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. CA IX의 저산소성 표지자로 연구용에 적합. Thermo Fisher Scientific 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Horse, Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 66.4.C2 (PN-15) |
| Immunogen | Microsomal fraction of human renal cortical tissue homogenate |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxia via HIF-1 alpha, due to its stability and membrane localization.
Carbonic anhydrases play a key role in pH regulation in normal tissues by controlling hydrogen ion (H⁺) flux.
Blockade of CA IX increases cell death under hypoxia, making CA IX a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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