Thermo Fisher Scientific Phospho-MKP-1 (Ser359) Polyclonal Antibody
Phospho-MKP-1 (Ser359) 폴리클로날 항체로, 인산화된 DUSP1/MKP1 단백질을 특이적으로 검출합니다. Western blot과 IHC(Paraffin) 실험에 적합하며, 사람과 생쥐 시료 모두에 반응합니다. 고순도 정제 및 안정적인 PBS/glycerol 보존 용액 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DUSP1 (Accession P28562), corresponding to amino acid residues around phosphorylated Ser359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816632 |
Product Specific Information
This antibody detects endogenous levels of DUSP1/MKP1 only when phosphorylated at Ser359.
Target Information
The DUSP1 gene is induced in human skin fibroblasts by oxidative or heat stress and growth factors. It encodes a protein similar to non-receptor-type protein-tyrosine phosphatases, showing sequence similarity to a Tyr/Ser-protein phosphatase encoded by the vaccinia virus late gene H1. DUSP1 protein exhibits intrinsic phosphatase activity, specifically inactivating mitogen-activated protein (MAP) kinase by dephosphorylating both phosphothreonine and phosphotyrosine residues. It also suppresses MAP kinase activation by oncogenic ras in Xenopus oocyte extracts, indicating its role in stress response and negative regulation of cellular proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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