Thermo Fisher Scientific Fibronectin Monoclonal Antibody (FN1, 2948)
Fibronectin 단백질을 인식하는 마우스 단클론 항체로, IHC(P) 및 Peptide Array에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, 4°C에서 보관. 세포 부착, 이동, 발달 및 암 연구에 적합한 연구용 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FN1, 2948 |
| Immunogen | Recombinant fragment (around aa 467–595) of human fibronectin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed):
- Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Fibronectin is a disulfide-bonded dimer (230–250 kDa) found in the extracellular matrix of connective tissues and vessels. It exists as an insoluble, cross-linked polymer and as a soluble plasma protein.
Fibronectin plays a key role in cell adhesion, migration, embryonic development, wound healing, coagulation, and metastasis.
Increased expression of fibronectin, especially in non-small cell lung carcinoma, enhances tumorigenicity and resistance to apoptosis.
The fibronectin gene undergoes alternative splicing, potentially producing up to 20 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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