Thermo Fisher Scientific Nod2 Monoclonal Antibody (26mNOD2), eBioscience

Applications and Publications

Product Specifications

Product Specific Information

The monoclonal antibody 26mNOD2 reacts with mouse NOD2.
NOD2, also known as caspase recruitment domain-containing protein 15 (CARD15), is a cytosolic protein (~115 kDa) containing:

• A central NOD (nucleotide oligomerization domain)
• A C-terminal leucine-rich repeat (LRR) domain
• An N-terminal CARD domain

The LRR domain recognizes microbial components such as muramyl dipeptide, while the CARD domain interacts with RIP2 via homotypic CARD-CARD interaction, leading to downstream activation of NF-κB.

This antibody recognizes protein from transgenic and transfected cells.
Applications reported include immunoblotting (WB), and it has been validated on transfected cell lines and NOD2-transgenic mouse cells.

Target Information

NOD2 is a member of the apoptosis-regulating protein family that includes Apaf-1 and NOD1, containing:

• Two N-terminal CARDs
• A nucleotide binding domain (NBD)
• Multiple C-terminal leucine-rich repeats (LRRs)

NOD2 is primarily expressed in monocytes, whereas NOD1 is expressed in multiple tissues.
It regulates NF-κB signaling, acts as an intracellular receptor for bacterial lipopolysaccharides, and is associated with inflammatory bowel disease (IBD) and Crohn’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.