Thermo Fisher Scientific RhoH Polyclonal Antibody
RhoH 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 고순도 및 높은 특이성을 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 102–152 of Human RhoH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719062 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. Members such as Rho, Rac, and CDC42 regulate actin reorganization and influence cellular adhesion, cytokinesis, and motility.
RhoH (also known as TTF or ras homolog gene family member H) differs from most small G proteins in that it is expressed primarily in hematopoietic cells and tissues. Translocations and frequent RhoH mutations have been detected in primary lymphoma cells, and expression is also observed in activated neutrophils.
RhoH is GTPase-deficient, remaining in a GTP-bound activated state without cycling, and may play a role in T-cell differentiation and cytoskeletal organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191-amino acid polypeptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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