Thermo Fisher Scientific Human BAFF (BLyS) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human BAFF, amino acids Ala134-Leu285 (Accession # Q9Y275)

Molecular weight

18.5 kDa

Class

Recombinant

Type

Protein

Purity

>90% by reducing SDS-PAGE

Endotoxin concentration

<0.40 ng/µg

Activity

ED50 = 1.6 µg/mL; determined by a mouse splenocyte survival assay.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Description: Recombinant human BAFF (B-cell activating factor), also referred to as BlyS, is a type II transmembrane protein of the TNF superfamily. BAFF is found on T cells, macrophages and dendritic cells. BAFF contains a cytoplasmic domain, transmembrane domain and extracellular domain which can be cleaved between amino acid 133 and 134 to produce a soluble form detectable in serum. Receptors for BAFF include TACI, BAMBI and BAFFR. BAFF/BLyS is a B lymphocyte stimulatory molecule; it induces B cell proliferation and immunoglobulin secretion. In addition BAFF can provide a co-stimulation signal to T lymphocytes. Elevated levels of BAFF has been implicated in the pathogenesis of some autoimmune diseases (particularly) B cell diseases such as systemic lupus erythaematosus. Human and mouse BAFF share 86% amino acid sequence identity.

Applications Reported: Recombinant human BAFF is biologically active.

Applications Tested: The ED50, as determined by a mouse splenocytes survival assay, is 1.6 µg/mL.

Source: E. coli expressed amino acids Ala134-Leu285 accession # Q9Y275.

Bioactivity: The ED50, as determined by a mouse splenocyte survival assay, is 1.6 µg/mL.

Endotoxin: Less than 0.40 ng/ug as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE.

Molecular Weight: 18.5 kDa.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.