Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), NovaFluor Yellow 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• Functional Grade
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

eBioTY/11.8 recognizes CD73, a 69-kDa GPI-anchored cell-surface protein with ecto-5’-nucleotidase activity.
CD73 expression on myeloid cells (CD11b) is restricted to bone marrow. In humans, CD73 can be induced to secrete a soluble form with IL-2, suggesting a role in activation signaling. Expression patterns differ between species: BALB/c mice show more CD4+CD73+ cells, while humans exhibit more CD8+CD73+ cells.

CD73 is expressed on a subset of lymphocytes and increases during lymphocyte maturation. Memory CD4 T cells expressing CD73 resemble uncommitted primed precursor helper cells (Thpp) that can differentiate into Th1 or Th2 cells. CD73 is also found on regulatory T cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.2 µg/test.
A test is defined as staining a 100 µL cell sample (10⁵–10⁸ cells/test). Optimize titration for best performance.

Compatibility Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

CellBlox Plus Blocking Buffer Usage:

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample before combining with cells
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells)

Technology:
NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures, engineered for minimal spillover and spread.

Excitation/Emission:

• Excitation: 552 nm
• Emission: 690 nm
• Laser: 561 nm (Yellow)

Strain-Specific Observation:
NovaFluor Yellow 755 shows non-specific B-cell staining in SJL mice.
No non-specific staining observed in BALB/c or C57BL/6 strains.

Target Information

CD73 (Ecto-5'-nucleotidase) catalyzes conversion of purine 5'-mononucleotides to nucleosides, preferring AMP.
It is a 70 kDa homodimer anchored to the plasma membrane via GPI linkage and serves as a marker of lymphocyte differentiation.
Deficiency of CD73 is associated with immunodeficiency diseases.
The CD73 gene is localized to chromosome 6q14–q21.
Defects can lead to joint and arterial calcification or intestinal tuberculosis.
Two transcript variants encoding different isoforms are known.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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