Thermo Fisher Scientific PPBP Polyclonal Antibody, PeproTech
Human NAP-2(CXCL7)를 인식하는 Rabbit Polyclonal Antibody로, Western Blot과 ELISA에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체. -20°C 보관, PBS buffer, preservative 없음. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P03-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PPBP Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| ELISA | 0.5–2.0 µg/mL | - |
| Immunostaining (IS) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human NAP-2 (CXCL7) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929135 |
Product Specific Information
AA Sequence of recombinant protein:
AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NAP-2 (CXCL7). Anti-Human NAP-2 (CXCL7)-specific antibody was purified by affinity chromatography employing an immobilized Human NAP-2 matrix.
Sandwich ELISA:
To detect Human NAP-2 (CXCL7) by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NAP-2 (CXCL7) (500-P03BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human NAP-2 (CXCL7).
Western Blot:
To detect Human NAP-2 (CXCL7) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human NAP-2 (CXCL7) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Members of the α-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif (Cys-X-Cys) and are chemotactic for neutrophils. The platelet basic protein (PBP), a member of the α-chemokine family, resides in the α-granules of platelets and is released upon activation. Proteolytic cleavage of the amino terminus of PBP generates several peptides, including mature PBP, connective tissue-activating peptide III (CTAP III, also known as low affinity platelet factor IV (LA-PF4)), β-thromboglobulin (β-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12–q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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