Thermo Fisher Scientific PPBP Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NAP-2 (CXCL7). Anti-Human NAP-2 (CXCL7)-specific antibody was purified by affinity chromatography employing an immobilized Human NAP-2 matrix.

Sandwich ELISA:
To detect Human NAP-2 (CXCL7) by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NAP-2 (CXCL7) (500-P03BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human NAP-2 (CXCL7).

Western Blot:
To detect Human NAP-2 (CXCL7) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human NAP-2 (CXCL7) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Members of the α-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif (Cys-X-Cys) and are chemotactic for neutrophils. The platelet basic protein (PBP), a member of the α-chemokine family, resides in the α-granules of platelets and is released upon activation. Proteolytic cleavage of the amino terminus of PBP generates several peptides, including mature PBP, connective tissue-activating peptide III (CTAP III, also known as low affinity platelet factor IV (LA-PF4)), β-thromboglobulin (β-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12–q13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.